Types of DNA Dye
The DNA dye may be primarily of THREE types
- The dye that binds in the minor groove of DNA
- The dye that is intercalated within the base pair
- Bis-intercalator
Important features often the three types of DNA dyes are summarised in the image below.
Basic Requirements of a Good DNA Dye
• Dye should be specific to DNA.
• Dye should bind stoichiometric to the DNA content.
• Dye should show strong enhancement of fluorescence after binding with
the target.
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